HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (29) Citing Articles via Google Scholar Google Scholar Articles by Murad, L. Articles by Leitch, A. R. Search for Related Content PubMed Articles by Murad, L. Articles by Leitch, A. R. Agricola Articles by Murad, L. Articles by Leitch, A. R.

The origin of tobacco's T genome is traced to a particular lineage within Nicotiana tomentosiformis (Solanaceae)¹

School of Biological Sciences, Queen Mary, University of London, London, E1 4NS, UK; Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ-61265 Brno, Czech Republic

Nicotiana tabacum (tobacco) is a natural allotetraploid. The maternal genome donor is not controversial and is probably derived from an ancestor of N. sylvestris. The paternal, T-genome donor has been less clear, with N. tomentosiformis, N. otophora, or an introgression hybrid proposed. Here we provide evidence that the T genome of N. tabacum is derived from a particular lineage of N. tomentosiformis. We show that the repetitive sequences of geminiviral origin, GRD53 and GRD3, are present in the genomes of N. tabacum cultivars, a tobacco cell suspension culture TBY-2, and N. tomentosiformis ac. NIC 479/84. Surprisingly, they are not present in another three varieties of N. tomentosiformis. A detailed cytogenetic analysis also revealed that N. tomentosiformis ac. NIC 479/84 most closely resembles the N. tabacum T genome in the location of other tandem repetitive sequences. Thus, tobacco formed after divergence within N. tomentosiformis, and the spectrum of potential donors of the paternal genome can be narrowed to a genotype of N. tomentosiformis characterized by the presence of GRD53 and GRD3 repeats. It is clear that future paternity studies in tobacco should use N. tomentosiformis ac. NIC 479/84 rather than any other accession.

Key Words: ancestors • evolution • geminivirus-related DNA • GRD • integration • Nicotiana • Solanaceae • tobacco

This article has been cited by other articles:

				P. J. Rushton, M. T. Bokowiec, S. Han, H. Zhang, J. F. Brannock, X. Chen, T. W. Laudeman, and M. P. Timko Tobacco Transcription Factors: Novel Insights into Transcriptional Regulation in the Solanaceae Plant Physiology, May 1, 2008; 147(1): 280 - 295. [Abstract] [Full Text] [PDF]

				L. B. Gavilano and B. Siminszky Isolation and Characterization of the Cytochrome P450 Gene CYP82E5v2 that Mediates Nicotine to Nornicotine Conversion in the Green Leaves of Tobacco Plant Cell Physiol., November 1, 2007; 48(11): 1567 - 1574. [Abstract] [Full Text] [PDF]

				L. B. Gavilano, N. P. Coleman, S. W. Bowen, and B. Siminszky Functional Analysis of Nicotine Demethylase Genes Reveals Insights into the Evolution of Modern Tobacco J. Biol. Chem., January 5, 2007; 282(1): 249 - 256. [Abstract] [Full Text] [PDF]

				N. Lannoo, W. J. Peumans, and E. J. M. Van Damme The presence of jasmonate-inducible lectin genes in some but not all Nicotiana species explains a marked intragenus difference in plant responses to hormone treatment J. Exp. Bot., September 1, 2006; 57(12): 3145 - 3155. [Abstract] [Full Text] [PDF]

				K. Y. Lim, K. Souckova-Skalicka, V. Sarasan, J. J. Clarkson, M. W. Chase, A. Kovarik, and A. R. Leitch A genetic appraisal of a new synthetic Nicotiana tabacum (Solanaceae) and the Kostoff synthetic tobacco Am. J. Botany, June 1, 2006; 93(6): 875 - 883. [Abstract] [Full Text] [PDF]

				M. Fojtova, A. Bleys, J. Bedrichova, H. Van Houdt, K. Krizova, A. Depicker, and A. Kovarik The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco. Nucleic Acids Res., January 1, 2006; 34(8): 2280 - 2293. [Abstract] [Full Text] [PDF]

				W. Gregor, M. F. Mette, C. Staginnus, M. A. Matzke, and A. J.M. Matzke A Distinct Endogenous Pararetrovirus Family in Nicotiana tomentosiformis, a Diploid Progenitor of Polyploid Tobacco Plant Physiology, March 1, 2004; 134(3): 1191 - 1199. [Abstract] [Full Text] [PDF]

				K. Skalicka, K. Y. Lim, R. Matyasek, B. Koukalova, A. R. Leitch, and A. Kovarik Rapid evolution of parental rDNA in a synthetic tobacco allotetraploid line Am. J. Botany, July 1, 2003; 90(7): 988 - 996. [Abstract] [Full Text] [PDF]