HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Taliercio, E. Articles by Stewart, J. McD. Search for Related Content PubMed Articles by Taliercio, E. Articles by Stewart, J. McD. Agricola Articles by Taliercio, E. Articles by Stewart, J. McD.

DNA content and expression of genes related to cell cycling in developing Gossypium hirsutum (Malvaceae) fibers¹

²USDA/ARS, Crop Genetics and Production Research Unit, 141 Experiment Station Road, Stoneville, Mississippi 38776 USA; ³University of Arkansas, CSES Department, University of Arkansas, Fayetteville, Arkansas 72701 USA

ABSTRACT

The cell cycle in cotton (Gossypium hirsutum) fibers is poorly understood. The objective of this study was to evaluate the cell cycle status and DNA content in developing cotton fibers. The DNA content and cell cycle distribution in fiber and hypocotyl cells were determined by flow cytometry. Expression levels of minichrosomal maintenance protein (mcm), cyclin B, and a retinoblastoma-like protein (rb) genes were determined with real-time PCR in fibers and dividing and nondividing tissues. No endoreduplication occurred, nor did genome size or percentage of G1-phase nuclei differ between hypocotyls and fibers. Approximately 13 and 17% of fiber nuclei were in the S phase in 14 days after anthesis (d) fibers and 25 d fibers, respectively. The mcm and cyclin B were expressed at higher levels in fibers than in mature leaves, but expression levels in fibers were less than 15% of meristematic tissues. Rb was expressed in fibers at levels less than 50% of mature leaves or meristematic tissues. Based on an apparent increase in S-phase cells as fibers mature and the low level of expression of genes associated with cell cycle progression, we conclude that S-phase arrest occurs in developing cotton fiber.

Key Words: • cyclin B • fiber DNA content • flow cytometry • Gossypium hirsutum • minichromsomal maintenance protein • Naked Seed mutant • retinoblastoma protein

This article has been cited by other articles:

				J. J. Lee, A. W. Woodward, and Z. J. Chen Gene Expression Changes and Early Events in Cotton Fibre Development Ann. Bot., December 1, 2007; 100(7): 1391 - 1401. [Abstract] [Full Text] [PDF]

				R. W. Shultz, V. M. Tatineni, L. Hanley-Bowdoin, and W. F. Thompson Genome-Wide Analysis of the Core DNA Replication Machinery in the Higher Plants Arabidopsis and Rice Plant Physiology, August 1, 2007; 144(4): 1697 - 1714. [Abstract] [Full Text] [PDF]